Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-379 inhibits proliferation and induces apoptosis in multiple myeloma by targeting Y-box binding protein 1

Yunyun Wang¹, Dexiang Ji², Guoan Chen²

¹Academic Affairs Office; ²Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province 330006, China.

For correspondence:- Guoan Chen Email: chenguoan0791@163.com

Accepted: 30 August 2020 Published: 30 September 2020

Citation: Wang Y, Ji D, Chen G. MiR-379 inhibits proliferation and induces apoptosis in multiple myeloma by targeting Y-box binding protein 1. Trop J Pharm Res 2020; 19(9):1843-1849 doi: 10.4314/tjpr.v19i9.7

© 2020 The authors.
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Abstract

Purpose: To determine the effect of miR-379 in multiple myeloma.

Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to evaluate the expression of miR-379 in multiple myeloma cells. The effect of miR-379 on multiple myeloma progression was investigated by cell counting, bromodeoxyuridine staining, flow cytometry and Western blot analysis. A potential target for miR-379 was determined using a luciferase reporter assay.

Results: MiR-379 expression was reduced in multiple myeloma cells, while over-expression of miR-379 increased both cell viability and proliferation of these cells (p < 0.05). Moreover, miR-379 blocked cell cycle multiple myeloma cells and promoted apoptosis by decreasing Bcl-2 expression, and increasing the expression of cleaved caspase-3 and Bax. MiR-379 bound to Y-box binding protein 1 (YBX1) and reduced YBX1 mRNA and protein expression in multiple myeloma cells (p < 0.05).

Conclusion: A YBX1-mediated tumor-suppressive role for miR-379 in multiple myeloma cells has been identified, suggesting a potential strategy for the treatment of multiple myeloma.

Keywords: MiR-379, Y-box binding protein 1, Multiple myeloma, Proliferation, Apoptosis